WebAnti-HA-Biotin, High Affinity (3F10) is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to biotin. The … WebBound anti-HA antibodies were subsequently detected using anti-mouse-peroxidase (in the case of clone 12CA5) and anti-rat-peroxidase (in the case of clone 3F10) and by chemiluminescence detection. SDS polyacrylamide gel electrophoresis, transfer onto a nylon membrane and subsequent detection were carried out as described in example 3.1 dolly cinema WebApril 25th, 2024 - Ha ha jeg istemmer at vi bor sammen hele gjengen så kan vi utnytte hverandres fordeler Du ville sørget for at vi alltid gjorde noe hyggelig sammen og at vi spiste alle måltider rundt et vakkert dekket bord og du er jo ikke akkuratt dårlig til å lage mat og ha det koselig rundt deg du heller Du hadde også passet på at ... WebMonoclonal Anti-Strep Tag antibody produced in mouse (monoclonal GT661, affinity isolated antibody); Suitable for western blot; Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: 1:100-1:500, WB: 1:1000-1:10000; Evaluate our antibodies with complete peace of mind; ... Anti-HA-Biotin, High Affinity (3F10) from rat IgG 1. View ... dolly clock WebChemical structure requirements and cellular targeting of microRNA-122 by peptide nucleic acids anti-miRs. Gait MJ Nucleic acids research 40.5 (2012 Mar): 2152-67. LAMP2 microdeletions in patients with Danon disease. Vatta M Circulation. Cardiovascular genetics 3.2 (2010 Apr): 129-37. WebJun 15, 2012 · Lysates were prepared as described 20 and incubated with anti-FLAG (M2, Sigma, St Louis, MO, USA) agarose beads. FH-CRBN was eluted with SDS–PAGE buffer and SDS–PAGE separated proteins immunoblotted with anti-HA antibody (3F10, Roche). Unless otherwise indicated, compounds were added to cells 3 h before addition of MG132. contemporary evening dresses WebHA tag stably transfected CHO cells were fixed with ice cold methanol for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1 µg/ml Alexa Fluor® 647 conjugated (red) anti-HA.11 Epitope Tag antibody (clone 16B12 ) in blocking buffer for two hours at room temperature.

WebMar 1, 2024 · Article Snippet: Sources (in parentheses) of primary antibodies are as follows: monoclonal rat anti-HA (3F10) (Roche), affinity purified rabbit anti-Kv11.1 against the … WebNo rating or validation information has been found for Anti-HA High Affinity; Rat monoclonal antibody (clone 3F10). ... (clone 3F10). Data and Source Information . Source: Antibody … contemporary european style homes Anti-HA High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10). Anti-HA High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique ... WebMar 30, 2024 · antibody: anti-HA, 3F10 Roche: Treatment protocol: EL4 cells were stimulated with phorbol 12-myristate 13-acetate (50 ng/ml) and ionomycin (1 μM) for 4 … dollycr8 WebConfocal immunofluorescent analysis of COS cells, mock-transfected (left) or transfected with HA-tagged protein (right), using HA-Tag (6E2) Mouse mAb (Alexa Fluor ® 488 Conjugate) #2350. Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ™ (fluorescent DNA dye). Show More. Web1 day ago · The total lysate supernatant was aliquot and incubated with an anti-HA antibody with a dilution of 1:50 (Clone BMG-3F10, Roche, Switzerland) or a Rat IgG control (sc-2006, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for overnight at 4 °C. The next day, each reaction was supplied with 40 μL protein A/G agarose for another 2 h at 4 °C. dolly clock timer WebThis antibody has been shown to work in applications such as: Precipitation, ChIP, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot. HA-Tag (C29F4) Rabbit mAb detects exogenously expressed proteins containing the HA epitope tag.

WebRB545 Mouse Anti-Mouse CD182 (CXCR2) Single Color Antibodies RUO Products. Instruments. ... CXCL5 (LIX), and CXCL7 (NAP-2). CD182 (CXCR2) plays a role in neutrophil activation and trafficking. The 3F10_B3 antibody was raised against Mouse CXCR2-transfected cells. RB545 Mouse Anti-Mouse CD182 (CXCR2) Clone 3F10_B3. … dolly cola WebJul 15, 2013 · 厂商Mat NoProductNamePackSize2011List Price说明书Roche11867423001Anti-HA High Affinity (3F10), 50 µg 3758https:… dolly coutinho leeds