Phm1 vector
Webb26 dec. 2024 · Therefore, we constructed a helper λ‐Red recombination system into the pHM1 vector, yielding pHM1‐λRed (Figure 3b), and transformed this into Xoo to improve … Webb1 maj 2012 · For immunofluorescence, 5 x 10 4 PHM1 cells expressing FNDC3B or control vector were plated on 0.2% gelatin coated coverslips (Corning No. 1½) in 6-well plates …
Phm1 vector
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WebbMany plant-associated bacteria produce plant- mimicking hormones which are involved in modulating host physiology. However, their function in modulating bacterial physiology … Webb12 aug. 2024 · Plasmid pHM1-XocRNA-CSpec1-CSpec2 targeting the spectinomycin resistant gene was transferred into PXO99 A cells and plated on spectinomycin-containing medium. Only 8 spectinomycin resistant transformants were obtained, but lots of colonies were observed to form when using the control vector ( Figure 1C ).
Webb1 apr. 2007 · The broad host-range vector pHM1 (Yang et al., 2000) was used in complementation experiments. Concentrations of the antibiotics used were as follows: …
Webbwas similarly constructed [29]. Every recombinant vector was linearized with HindIII and cloned into the pHM1 vector for genetic complementation. The hpa1pthXo1 and … soft touch foam bitWebb12 juli 2024 · Taq polymerase ReadyMix (KAPA Biosystems, Wilmington, MA, USA) was used for all screening purposes. For cloning in the pHM1 vector (Innes et al., 1988), primers as listed in Table S2 were used for amplification of the xopX gene and its 14-3-3 protein-binding motif mutants using Phusion polymerase (Thermo Fisher Scientific). soft touch felt padsWebbVectors Used in Methanotrophs 4.2 Gene Transfer The most common mode of gene transfer for methanotrophs is conjugation, although electroporation has also been successful in Type II methanotrophs (Table 4.2). The one report of transformation ( Williams and Bainbridge, 1971) has not been repeated. 4.2.1. Conjugation Protocols. soft touch finishWebbpHom-1. Mammalian vector encoding an FKBP homodimerizer domain, for creating soluble fusion proteins that can be dimerized with a drug. Also known as pC4-FV1E. Sequence … soft touch grip padsWebb4 sep. 2024 · Every recombinant vector was linearized with HindIII and cloned into the pHM1 vector for genetic complementation. The hpa1pthXo1 and hpa1avrXa10 double … soft touch foot peel maskhttp://biovector.net/product/319420.html softtouch furniture glides swivelWebbpHM was a gift from Markus Ralser (Addgene plasmid # 64173 ; http://n2t.net/addgene:64173 ; RRID:Addgene_64173) For your References section: … soft touch gun stock recoil system